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cleaved parp  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology cleaved parp
    Cleaved Parp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 2148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cleaved+parp/cleaved+PARP-1+Antibody/pm42879957-431-10-22
    Average 96 stars, based on 2148 article reviews
    cleaved parp - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Lysis:

    Article Title: Targeting DHODH reveals a metabolic vulnerability in AR-positive and AR-negative prostate cancer cells via pyrimidine synthesis and metabolic crosstalk with the TCA and urea cycles
    Article Snippet: .. Lysis buffer K was made with 20 mM Na 2 HPO 4 , 0.15 M NaCl, 0.1% NP40, 5 mM EDTA, and CHAPS 0.01 % proteinase inhibitor (Sigma–Aldrich #P8340-5 ML) and a phosphatase inhibitor cocktail tablet (Roche#04-906-837-001) was added for 10 mL of Lysis buffer K. For the apoptosis signaling, western blot analysis was performed with primary antibodies DHODH (Cell Signaling Technology #26381), cleaved PARP (Santa Cruz Biotechnology #sc-56196), α-tubulin (Cell Signaling Technology #2125); ribosomal protein S6 (Santa Cruz Biotechnology #sc-74459); and AR statut (Santa Cruz Biotechnology #sc-7305). ..

    Western Blot:

    Article Title: Targeting DHODH reveals a metabolic vulnerability in AR-positive and AR-negative prostate cancer cells via pyrimidine synthesis and metabolic crosstalk with the TCA and urea cycles
    Article Snippet: .. Lysis buffer K was made with 20 mM Na 2 HPO 4 , 0.15 M NaCl, 0.1% NP40, 5 mM EDTA, and CHAPS 0.01 % proteinase inhibitor (Sigma–Aldrich #P8340-5 ML) and a phosphatase inhibitor cocktail tablet (Roche#04-906-837-001) was added for 10 mL of Lysis buffer K. For the apoptosis signaling, western blot analysis was performed with primary antibodies DHODH (Cell Signaling Technology #26381), cleaved PARP (Santa Cruz Biotechnology #sc-56196), α-tubulin (Cell Signaling Technology #2125); ribosomal protein S6 (Santa Cruz Biotechnology #sc-74459); and AR statut (Santa Cruz Biotechnology #sc-7305). ..

    Incubation:

    Article Title: Glucagon-like peptide-1 receptor (GLP-1R) agonists prevent tributyltin-induced muscle atrophy/wasting via restoring GLP-1R signaling in vitro and in mice.
    Article Snippet: .. Membranes were blocked with 5 % skim milk for 1 h at room temperature, followed by overnight incubation at 4◦C with the following primary antibodies: atrogin-1 (1:1000), FoxO1A (1:1000), cleaved PARP (1:1000), and myostatin (1:1000) (Abcam, MA, USA); MuRF-1 (1:1000), Bcl-2 (1:1000), PARP (1:1000), GLP-1R (1:1000), p38 (1:1000), phosphorylated ERK (1:1000), ERK (1:1000), and β-actin (1:1000) (Santa Cruz Biotechnology, Santa Cruz, CA, USA); Bax (1:1000), cleaved caspase-3 (1:1000), caspase-3 (1:1000), phosphorylated FoxO1A (1:1000), AMPKα (1:1000), phosphorylated AMPKα (1:1000), phosphorylated p38 (1:1000), JNK (1:1000), and phosphorylated JNK (1:1000) (Cell Signaling Technology, Danvers, MA, USA); and Na+/K+ ATPase α (1:1000) (Epitomics, CA, USA), overnight at 4◦C. ..

    other:

    Article Title: HJ-4, a novel piperine derivative, inhibits tumor growth and angiogenesis via p53 activation and oncogenic pathway inhibition in colorectal cancer models
    Article Snippet: Equal amounts of protein (30–50 μg) were loaded onto 10–12% SDS-polyacrylamide gels, separated by electrophoresis, and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA) using a Bio-Rad wet transfer system at 100 V for 90 min. Membranes were blocked with 5% non-fat milk in TBS-T buffer (Tris-buffered saline containing 0.1% Tween-20) for 1 h at room temperature and incubated overnight at 4 °C with primary antibodies diluted in blocking buffer.



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